Expression levels of plasma exosomal miR-124, miR-125b, miR-133b, miR-130a and miR-125b-1-3p in severe asthma patients and normal individuals with emphasis on inflammatory factors

Abstract Background Identification of molecular markers, such as miRNAs is promising for the diagnosis asthma and its clinical phenotypes. The aim this study was to examine changes in expression selected microRNAs plasma exosomal fractions severe patients. determined relation inflammatory markers. Method Severe patients (n = 30) healthy subjects were among individuals referred allergy clinic. Blood collected from each participant determine serum high-sensitive C-reactive protein (hs-CRP) total IgE. fraction isolated processed quantitation miR-124, miR-125b, miR-133b, miR-130a miR-125b-1-3p using quantitative real time-PCR (qRT-PCR). Results Serum hs-CRP IgE significantly higher compared controls. Expression down-regulated controls (p < 0.0001). However, miR-125b substantially There no significant difference Data analysis revealed that miRNAs, highly correlated with levels Conclusion Overexpression which associated may suggest molecule linked reactions. Up-regulation together decreased miRNA profile useful discrimination phenotypes asthma.